Drug release systems based on nanotechnology reduce side effects, increase drug release time, and control drug release. Polymeric nanoparticles that are used as pharmaceutical nanocarriers lead to increasing the solubility and stability of drugs.Increasing concerns about the safety of chemical drugs and additives has caused a demand for the use of bioactives. Drug concentration is one of the important factors for investigating cytotoxicity, which can play a role in cell death and anti proliferation. Therefore, effective drug release by nanocarriers eliminates poor and unstable solubility in cells. The designed nanocarrier causes long-term drug release in the cancerous tumor environment, which, as a result, increases cytotoxicity. In most of the new researches, nanocarriers were made by double emulsion method, which makes the shape of nanoparticles stable and uniform and also prevents the acceleration of nanoparticles during the drug release process. In this research, the cytotoxicity analysis of new nanocarriers made in breast cancer cell line has been analyzed.