دنیای نانو

دنیای نانو

بهبود زیست‌فراهمی و اثربخشی زیستی پونیکالاژین و اسید آلاژیک پوست انار با استفاده از فناوری نانو: یک مرور جامع

نوع مقاله : مروری

نویسندگان
1 گروه علوم و مهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران
2 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه گیلان
چکیده
پوست انار منبع مهمی از ترکیبات فنولی مثل پونیکالاگین، اسید الاجیک است که خواص دارویی و غذایی قابل توجهی دارند. با این حال، یکی از مشکلات اصلی این ترکیبات، زیست‌فراهمی پایین آن‌ها و کاهش اثر در مسیر گوارشی است. در این مقاله مروری، ابتدا ویژگی‌های شیمیایی و اثرات بیولوژیکی این مواد بررسی شده و سپس نقش فناوری‌های نانو در بهبود جذب و پایداری آن‌ها مطرح شده است. شواهد نشان می‌دهد که استفاده از روش‌های نانو، مانند نانوکپسوله‌سازی و حامل‌های نانو، می‌تواند اثرات درمانی و قابلیت دسترسی زیستی این ترکیبات را افزایش دهد و بکار گیری از تکنیک فناوری نانو مسیرهای تازه‌ای برای ارتقای کاربردهای آن باز می‌کند. استفاده از فناوری‌های نانو، شامل نانوفرموله‌ها، نانوذره‌ها و سیستم‌های تحویل هدفمند، نشان داده است که می‌توان این محدودیت‌ها را کاهش داده و پایداری، جذب روده‌ای و اثرات بیولوژیکی این ترکیبات را به طور قابل توجهی بهبود بخشید. چشم‌انداز تحقیقات آینده باید بر توسعه استراتژی‌های نوآورانه نانوفرموله با قابلیت کنترل رهایش، هدف‌گیری بافتی و افزایش بیوفراهمی متمرکز هستند، بر این اساس، مطالعات مکانیزمی و بالینی برای ارزیابی ایمنی، اثربخشی و تعاملات سیستمی این ترکیبات در مدل‌های انسانی ضروری بنظر خواهد رسید.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Improving the bioavailability and health-related applications of punicalagin and ellagic acid from pomegranate peel with nanotechnology approaches

نویسندگان English

Khorshid Nikkhou 1
Abbas Abedfar 2
1 Department of Food Science and Technology, Agriculture Faculty, University of Guilan, Rasht, Iran.
2 Department of Food Science & Technology, University of Guilan, Rasht, Iran.
چکیده English

Pomegranate peel is an important source of phenolic compounds such as punicalagin, ellagic acid, which have significant medicinal and nutritional properties. However, one of the main problems of these compounds is their low bioavailability and reduced effect in the digestive tract. In this article, first, the chemical properties and biological effects of these substances are reviewed and then the role of nanotechnology in improving their absorption and stability is discussed. Evidence shows that the use of nano methods, such as nanoencapsulation and nanocarriers, can increase the therapeutic effects and bioavailability of these compounds, and the use of nanotechnology techniques opens new avenues for improving their applications.In fact the use of nanotechnology including nanoformulations, nanoparticles and targeted delivery systems, has shown that these limitations can be reduced and the stability, intestinal absorption, and biological effects of these compounds can be significantly improved. Future research perspectives should focus on developing innovative nanoformulation strategies with controlled release, tissue targeting, and enhanced bioavailability. Also, mechanistic and clinical studies are necessary to evaluate the safety, efficacy, and systemic interactions of these compounds in human models.

کلیدواژه‌ها English

Punicalagin
Biodegradability
Nanoformulation
Nanotechnology
Health-related Properties
[1] Yuan Z, Fang Y, Zhang T, Fei Z, Han F, Liu C, Liu M, et al. The pomegranate (Punica granatum L.) genome provides insights into fruit quality and ovule developmental biology. Plant Biotechnol J. 2018; 16(7): 1363-74. doi:10.1111/pbi.12879.
[2] Yang R, Li J, Cheng G, Inta A, Yang L. Textiles dyeing with pomegranate (Punica granatum) peel extract using natural mordant. J Nat Fibers. 2023; 20(2). doi:10.1080/15440478.2023.2282056.
[3] Ko K, Dadmohammadi Y, Abbaspourrad A. Nutritional and bioactive components of pomegranate waste used in food and cosmetic applications: A review. Foods. 2021; 10(3): 657. doi:10.3390/foods10030657.
[4] Ge S, Duo L, Wang J, GegenZhula, Yang J, Li Z, Tu Y. A unique understanding of traditional medicine of pomegranate peel: A review. Front Pharmacol. 2021; 12: 688367.
[5] Derakhshan Z, Ferrante M, Trovato M, Conti GO, Alfio AK, Jeitner TM, et al. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juices and seeds. Food Chem Toxicol. 2018; 114: 108–115.
[6] Akhtar S, Ismail T, Fraternale D, Sestili P. Pomegranate peel and peel extracts: Chemistry and food features. Food Chem. 2015; 174: 417–425.
[7] Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol. 2012; 143(2): 397–405.
[8] Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol. 2007; 109(2): 177–206.
[9] Viuda-Martos M, Fernández-López J, Pérez-Álvarez JA. Pomegranate and its many functional components as related to human health: A review. Compr Rev Food Sci Food Saf. 2010; 9(6): 635–654.
[10] Colombo E, Sangiovanni E, Dell’Agli M. A review on the anti-inflammatory activity of pomegranate in the gastrointestinal tract. Evid Based Complement Alternat Med. 2013; 2013: 247145.
[11] Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M. A review study on Punica granatum L. J Evid Based Complementary Altern Med. 2016; 21(3): 221–227.
[12] Peels with the Aid of Ultrasound-Assisted Alkaline Hydrolysis. Molecules. 2024; 29(11): 2424. doi:10.3390/molecules29112424.
[13] Zuccari G, Baldassari S, Ailuno G, Turrini F, Alfei S, Caviglioli G. Formulation Strategies to Improve Oral Bioavailability of Ellagic Acid. Appl. Sci. 2020; 10(10): 3353. doi:10.3390/app10103353.
[14] Ramlagan P, Labib RM, Farag MA, Neergheen VS. Advances towards the analysis, metabolism and health benefits of punicalagin, one of the largest ellagitannin from plants, with future perspectives. Phytomed Plus. 2022; 2(3): 100313. doi:10.1016/j.phyplu.2022.100313.
[15] Huang Z, Foo SC, Choo WS. A review on the extraction of polyphenols from pomegranate peel for punicalagin purification: Techniques, applications, and future prospects. Sustain Food Technol. 2025; 3(2): 396-413.
[16] Kaderides K, Papaoikonomou L, Serafim M, Goula AM. Microwave-assisted extraction of phenolics from pomegranate peels: Optimization, kinetics, and comparison with ultrasound extraction. Chem Eng Process. 2019; 137: 1-11. doi:10.1016/j.cep.2019.01.006.
[17] García P, Fredes C, Cea I, Lozano-Sánchez J, Leyva-Jiménez FJ, Robert P, Vergara C, Jimenez P. Recovery of bioactive compounds from pomegranate (Punica granatum L.) peel using emerging technologies. Molecules. 2021; 26(6): 1614.
[18] Qu W, Breksa AP, Pan Z, Ma H. Quantitative determination of major polyphenol constituents in pomegranate peel. Food Chem. 2012; 132(3): 1585–1591.
[19] Fischer UA, Carle R, Kammerer DR. Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MSn. Food Chem. 2011; 127(2): 807–821.
[20] Larrosa M, García-Conesa MT, Espín JC, Tomás-Barberán FA. Ellagitannins, ellagic acid and vascular health. Mol Aspects Med. 2010; 31(6): 513–539.
[21] Landete JM. Ellagitannins, ellagic acid and their derived metabolites: A review about source, metabolism, functions and health. Food Res Int. 2011; 44(5): 1150–1160.
[22] Cerdá B, Espín JC, Parra S, Martínez P, Tomás-Barberán FA. The potent in vitro antioxidant ellagitannins from pomegranate juice are metabolised into bioavailable glucuronides of ellagic acid and urolithin in humans. Eur J Nutr. 2004; 43(4): 205–220.
[23] González-Sarrías A, Giménez-Bastida JA, García-Conesa MT, Gómez-Sánchez MB, García-Talavera NV, et al. Occurrence of urolithins, gut microbiota ellagic acid-derived metabolites, in human colorectal tissue. Mol Nutr Food Res. 2016; 60(10): 2238–2248.
[24] Tomás-Barberán FA, González-Sarrías A, García-Villalba R, Núñez-Sánchez MA, Yáñez-Gascón MJ, et al. Urolithins, the microbial metabolites of dietary ellagitannins, exhibit potent antioxidant, anti-inflammatory and antiproliferative activities. J Agric Food Chem. 2014; 62(29): 6585–6603.
[25] Espín JC, Larrosa M, García-Conesa MT, Tomás-Barberán FA. Biological significance of urolithins, the gut microbial ellagic acid-derived metabolites: The evidence so far. Mol Nutr Food Res. 2013; 57(7): 1179–1196.
[26] Bialonska D, Ramnani P, Kasimsetty SG, Muntha KR, Gibson GR, Ferreira D. The influence of pomegranate fruit extract in comparison to its isolated constituents on human gut microbiota. Food Chem. 2010; 119(3): 1192–1198.
[27] Kang I, Guo T, Patel M, Yang X. Ellagic acid and its microbial metabolite urolithin A alleviate obesity and associated complications. Mol Nutr Food Res. 2016; 60(8): 1772–1784.
[28] Xu Y, Shi C, Wu Q, Zheng Z, Liu P, Li G, Peng X, Xia X. Antimicrobial activity of punicalagin against Staphylococcus aureus and its effect on biofilm formation. Foodborne Pathog Dis. 2017; 14(5): 282-7. doi:10.1089/fpd.2016.2207.
[29] Azmat F, Safdar M, Ahmad H, Khan MRJ, Abid J, Naseer MS, Aggarwal S, et al. Phytochemical profile, nutritional composition of pomegranate peel and peel extract as a potential source of nutraceutical: A comprehensive review. Food Sci Nutr. 2024; 12(2): 661-74. doi:10.1002/fsn3.3700.
[30] Evtyugin DD, Magina S, Evtuguin DV. Recent Advances in the Production and Applications of Ellagic Acid and Its Derivatives. A Review. Molecules. 2020; 25(12): 2745. doi:10.3390/molecules25122745.
[31] Sanz M, Cadahía E, Esteruelas E, Muñoz AM, Fernández de Simón B, Hernández T, Estrella I. Phenolic compounds in chestnut (Castanea sativa Mill.) heartwood. Effect of toasting at cooperage. J Agric Food Chem. 2010; 58(17): 9631-40. doi:10.1021/jf101226w.
[32] Illescas-Montes R, Rueda-Fernández M, González-Acedo A, Melguizo-Rodríguez L, García-Recio E, Ramos-Torrecillas J, García-Martínez O. Effect of punicalagin and ellagic acid on the proliferation and differentiation of human osteoblasts. Food Funct. 2021; 12(14): 6325–6334.
[33] Seeram NP, Lee R, Heber D. Bioavailability of ellagitannins following consumption of pomegranates by humans. Clin Chim Acta. 2004; 348(1-2): 63–68.
[34] Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L, Derendorf H. Absorption, metabolism, and antioxidant effects of pomegranate (Punica granatum L.) polyphenols after ingestion of a standardized extract in healthy human volunteers. J Nutr. 2006; 136(10): 2481-5. doi:10.1093/jn/136.10.2481.
[35] Soares S, Mateus N, de Freitas V. Interaction of different classes of salivary proteins with food tannins. Food Res Int. 2012; 49(2): 807-13. doi:10.1016/j.foodres.2012.09.016.
[36] Grgić J, Šelo G, Planinić M, Tišma M, Bucić-Kojić A. Role of the encapsulation in bioavailability of phenolic compounds. Antioxidants. 2020; 9(10): 923. doi:10.3390/antiox9100923.
[37] Rahul RT, Dash KK, Sharma M. Recent advances in encapsulation of pomegranate peel extract and combination of wall materials: A review of encapsulation technologies, characterization and applications in the food industry. Sustain Food Technol. 2025. doi:10.1039/d4fb00196f.
[38] Ceci C, Graziani G, Faraoni I, Cacciotti I. Strategies to improve ellagic acid bioavailability: From natural or semisynthetic derivatives to nanotechnological approaches based on innovative carriers. Nanotechnology. 2020; 31(38): 382001. doi:10.1088/1361-6528/aba2e2.
[39] Pateiro M, Gómez B, Munekata PES, Barba FJ, Putnik P, Bursać Kovačević D, Lorenzo JM. Nanoencapsulation of promising bioactive compounds to improve their absorption, stability, functionality and the appearance of the final food products. Molecules. 2021; 26(6): 1547. doi:10.3390/molecules26061547.
[40] Mady FM, Shaker MA. Enhanced anticancer activity and oral bioavailability of ellagic acid through encapsulation in biodegradable polymeric nanoparticles. Int J Nanomedicine. 2017; 12: 7405-17. doi:10.2147/IJN.S140458.
[41] Zuccari G, Baldassari S, Ailuno G, Turrini F, Alfei S, Caviglioli G. Formulation strategies to improve oral bioavailability of ellagic acid. Appl Sci. 2020; 10(10): 3353. doi:10.3390/app10103353.
[42] Abd-Rabou AA, Shalby AB, Kotob SE. Newly synthesized punicalagin ellagitannin nanoparticles induce breast cancer cytotoxicity through ROS-mediated apoptosis. Asian Pac J Cancer Prev. 2022; 23(1): 363-76. doi:10.31557/APJCP.2022.23.1.363.
[43] Abd-Rabou AA, Shalby AB, Kotob SE. An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells. Cell Biochem Biophys. 2023; 81(2): 313-23. doi:10.1007/s12013-022-01083-x.
[44] McClements DJ, Xiao H. Designing food structure and composition to enhance nutraceutical bioactivity to support cancer inhibition. Semin Cancer Biol. 2017; 46: 215-26. doi:10.1016/j.semcancer.2017.06.012.
[45] Mawazi SM, Ge Y, Widodo RT. Niosome preparation techniques and structure—An illustrated review. Pharmaceutics. 2025; 17(1): 67. doi:10.3390/pharmaceutics17010067.
[46] Abd-Elghany AA, Mohamad EA. Chitosan-coated niosomes loaded with ellagic acid present antiaging activity in a skin cell line. ACS Omega. 2023; 8(19): 16620-9. doi:10.1021/acsomega.3c01097.
[47] Dundar AN, Ozdemir S, Uzuner K, Parlak ME, Sahin OI, Dagdelen AF, Saricaoglu FT. Characterization of pomegranate peel extract loaded nanophytosomes and the enhancement of bio-accessibility and storage stability. Food Chem. 2023; 398: 133921. doi:10.1016/j.foodchem.2022.133921.
[48] Ayachit AM, Patel VG. Self nanoemulsifying drug delivery system of stabilized ellagic acid–phospholipid complex with improved dissolution and permeability. Saudi Pharm J. 2015; 23(3): 276-89. doi:10.1016/j.jsps.2014.11.002.
[49] Zheng D, Lv C, Sun X, Wang J, Zhao Z. Preparation of a supersaturatable self-microemulsion as drug delivery system for ellagic acid and evaluation of its antioxidant activities. J Drug Deliv Sci Technol. 2019; 53: 101209. doi:10.1016/j.jddst.2019.101209.
[50] Sabu C, Shirin VKA, Sankar R, Pramod K. Inorganic nanoparticles for drug-delivery applications. In: Nanomaterials for Drug Delivery and Therapy. 2019; pp. 139–174.
[51] Unnikrishnan J, Hegde M, Girisa S, Satpati P, Kunnumakkara AB. Ellagic acid-gold nanoparticle complex as anticancer agent in triple-negative breast cancer cells. Nano Life. 2024. doi:10.1142/S179398442450003X.
[52] Mashkoor NR, Heydarnezhad M, Aghabali T, Ghaderi F, Sangani H, Nikoujamal N, Hedayati M, et al. Ellagic acid-conjugated iron oxide nanoparticles inhibit the cell cycle in the G0/G1 phase and induce extrinsic apoptosis in gastric cancer cell line. Chem Biodivers. 2025; 22(6): e202402294. doi:10.1002/cbdv.202402294.
[53] Nirwana I, Munadziroh E, Yuliati A, Fadhila AI, Nurliana, Wardhana AS, Shariff KA, Surboyo MD. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res. 2022; 12(1): 116–20. doi:10.1016/j.jobcr.2022.03.004.
[54] Iantomasi T, Palmini G, Romagnoli C, Donati S, Miglietta F, Aurilia C, Falsetti I, Marini F, Giusti F, Brandi ML. Dietary polyphenols and osteoporosis: Molecular mechanisms involved. Int J Bone Fragility. 2022; 2(3): 97–101. doi:10.1002/ijbf.2022009.
[55] Li Z, Percival SS, Bonard S, Gu L. Fabrication of nanoparticles using partially purified pomegranate ellagitannins and gelatin and their apoptotic effects. Mol Nutr Food Res. 2011; 55(7): 1096–103. doi:10.1002/mnfr.201000587.
[56] Hashim AJ, Alirezaei M, Soltani M, Es-haghi A, Minai-Tehrani D. Synthesis and characterization of albumin nanoparticles loaded by ellagic acid and chitosan and investigation of its anticancer activity against liver cancer cells. BioNanoScience. 2025; 15(1). doi:10.1007/s12668-025-01822-9.
[57] Rezagholizade-Shirvan A, Soltani M, Shokri S, Radfar R, Arab M, Shamloo E. Bioactive compound encapsulation: Characteristics, applications in food systems, and implications for human health. Food Chem X. 2024; 24: 101953. doi:10.1016/j.fochx.2024.101953.
دوره 21، شماره 79
تابستان 1404
صفحه 94-113

  • تاریخ دریافت 04 شهریور 1404
  • تاریخ بازنگری 05 آبان 1404
  • تاریخ پذیرش 26 آبان 1404