The threat of antibiotic drugs to humans and the environment has been noticed more than ever. Ciprofloxacin (Cip), as a strong quinolone antibiotic, is widely used in various treatments. This drug is among the fluoroquinolone drugs detected in surface water more often, which will bring health risks to animals and humans. In this study, a bimetallic metal-organic framework fluorescence nanosensor with d-f orbitals of intermediate metal and lanthanide with the molecular formula {[EuZn(pzdc)2(H2O)3]·H2O}n (MOF-I) (H2pzdc = 2,3-pyrazine dicarboxylic acid) was synthesized using the biocompatible method of ultrasonic waves to identify Cip antibiotic. Structure and characteristics of MOF-I nanosensor by powder X-ray diffraction (PXRD), Fourier transform infrared (FT-IR), thermal analysis (TGA), photoluminescence (PL) spectra, ultraviolet-visible (UV) spectrophotometry -Vis) and scanning electron microscope (SEM). According to the red emission of MOF-I caused by 4f-5d transitions of central metal Eu3+ corresponding to energy levels 5D0→7FJ (J=0→4), it was investigated to identify Cip. The experimental results show that the red fluorescence emission of the MOF-I nanosensor is turned off in the presence of the Cip molecule and the gradual increase in its concentration, using the internal filter effect (IFE) mechanism. The MOF-I nanosensor is associated with fast, stable, selectable response and high sensitivity in determining Cip. Considering the good linear correlation of the fluorescence response of MOF-I nanosensor to Cip concentration in the range of 1 to 40 μM and the lowest LOD equal to 0.32 μM, it indicates the reliability and applicability of the synthetic nanosensor in the detection of Cip antibiotic. .